banana dna extraction lab report conclusion

The conical flasks was labelled S and SS. Seeing Banana Banana DNA Extraction Activity Ask A Biologist activity for classroom and home Table of Contents Activity Overview Materials Review of Steps and What is Happening Instruction sheet For Teachers Next Generation Science Standards, Arizona Standards, xb``a``f Y800* @1*?./j D. student in bioinformatics at the University of Georgia. It does not store any personal data. These instructions are in segments of DNA called genes. with free plagiarism report. 1 2 . Studying DNA is one way scientists learn about what is necessary for life. 0000004908 00000 n Then the DNA can be precipitated, or come out of the solution, by adding alcohol. The remaining two bases, cytosine and thymine, are known as pyrimidines. Banana DNA Extraction by aaliyah jones - Prezi 0. g of salt was added into flask SS. Extracting DNA from Bananas In the Lab: Extracting DNA from Bananas, DNA was removed from bananas that had been blended with water in order to examine how DNA is seen from the naked eye. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity. Hypothesis: All living things including humans, plants (bananas!) 166 0 obj <>stream We even have genes in common with the banana plant! The DNA precipitates out of the solution when the alcohol is added. << Thus, DNA is present in all living organisms. PDF Banana DNA Extraction - Fleet Science Center 0000002514 00000 n The test tubes were carefully handled to avoid shaking. This process was repeated for 4 times. But opting out of some of these cookies may affect your browsing experience. endobj you to an academic expert within 3 minutes. Lastly, when good results were obtained, there was enough DNA to spool onto a rod or a plastic loop. 0000007612 00000 n Stir gently trying to avoid making too many bubbles in the mixture. Science at home: How to extract DNA from a banana 5. The probable reason for the tinted brown shade in the human epithelial DNA and not the strawberry DNA is that human epithelial DNA is extracted from one's mouth in which. Lab Report Banana Dna, Sample of Essays - EduCheer! 6T3G]Zq`S;z*H27$21a4a@ aaSn;,8@ U Variants of genes, called alleles, are responsible for differences in hair color, eye color, and earlobe shape. Once extracted, DNA could be observed and inferences made about the nature of DNA, and then compare the differences between two DNA's amount. The solution was stirred well and slowly. The information in DNA tells our bodies how to develop, grow, and work. Each strand is made up of a chain of nucleotides. * To investigate the effect of temperature on DNA extraction from bananas. /Type /XObject 1 0 obj It also controls many of the features that make an organism unique. Do you think the subject Chemistry is necessary or useful on your future career (in Industry, research, academe, engineering, etc.)? Pour the saltwater mix into the bag. The article Whole-Genome Patterns of Common DNA Variations in Three Human Populations provides detailed and examination of core individual differences in DNA sequences. You also have the option to opt-out of these cookies. In this activity, you will extract DNA from banana, strawberry and kiwi fruits. The filtration step (pouring the mixture through the strainer) allows for the collection of the DNA and other cellular substances. This is the DNA pieces clumping together. Order custom essay Extracting Dna from Bananas The filtrate, or filtered solution from the bag, was then placed into a test tube. For example, scientists can identity genetic cell membranes if the cell wall guards the cell, DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. &t #s, #"cre%#b$y sma$$' but we ca" see #t by extract#"g DN a"%, %#st#$$e% water #" the p$ast#c bag' sea$ the bag a"%, so$ut#o" s$ow$y !or about a m#"ute u"t#$ the shampoo, the empty cup. 0000002685 00000 n JFIF d d C DNA is in every living thing (and it's only in living things). Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. 0000045399 00000 n Extraction solution 'S' was added into ziplock 'Extraction 1' and extraxtion solution 'SS' into ziplock bad . << Using the wooden coffee stirrer, the DNA can be spooled out of the solution. (PDF) DNA Extraction Lab Report - ResearchGate Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . We are able to observe the extraction of genomic DNA from plant cells. In this biochemistry science project you will make your own DNA extraction kit from household materials and use it to purify DNA from strawberries. Melissa Wilson Sayres. harmony in order to life, Extracting Dna from Bananas. What is the purpose of extracting DNA from banana? /Filter /DCTDecode Genetic testing can best be defined as a process in which an individuals DNA is isolated and tested for the presence of specific genes or defects that could indicate the future onset of some disease. Explore how the human body functions as one unit in 6 : Group Bs Results Tan 1 1. Some additional protocols for DNA extractions: Sex Influenced Female Dominant Inheritance, beakers 100ml and 2x250ml (or jars that same size), liquid dishwashing detergent (clear solution works better than cloudy), Make a 10% solution of dishwashing detergent in one of your 250ml beakers (make enough to fill the mortar half way), Add the 10% detergent solution to cover the fruit, Grind the banana in the soap solution until there are no lumps (this is the hardest part, have the students take turns), Fasten a coffee filter to the 250 ml beaker with a rubber band so there is room for the fruit to be poured into the filter, Pour the fruit and soap mixture into the filter and place in a cold place to filter, Clean up your lab area while you are waiting for the filtering to complete, Remove the filter and throw away the contents, Pour enough of the liquid into a test tube to fill it 2 cm high, Add twice the volume of 95% ethanol (try to layer the alcohol on top by gently pouring it down the side of the test tube), The DNA will precipitate in the boundary between the alcohol and the banana mixture as a cloudy mass. cite it. DNA can be extracted and observed. Let us help you get a good grade on your paper. 0000006862 00000 n /Creator ( w k h t m l t o p d f 0 . For more info, see, Modern Language Association, 7th Ed. My 0000040445 00000 n DNA is organized into genes and stores genetic information. Ef+\:IZy}r(| F @q!4}g' U-CBb8sxurgg$t@AumUg Sx}Pa=cWyyh>WikxqB(;I&dCf&7CgCr(zMQl`PL BXIfBk. 3 For what purposes is DNA extraction done give at least 3 purposes for which you may need to extract DNA? Pour the mix into the filter and let it sit until all of the liquid drips down into the cup. DNA extraction The concentrations of the extracted DNA was measured using the NanoPhotometer,UV/Vis spectrophotometer (Implen, munich, Germany) and the average concentration for 12 samples was 0.045g/l. % You can use it as an example when writing << Result Photo 1: Test tubes containing solution S and SS CONCLUSION. Altering Animals. The total number of DNA for each fruit had a big variation between strawberries compared to kiwis and bananas with a difference of 5 and 6. every $#v#"g orga"#sm. 4 0 obj By clicking Accept All, you consent to the use of ALL the cookies. 3g PROCEDURE (A) Extraction solution recipe: 4ml of shampoo was mixed with 36ml of distilled water. How do I choose between my boyfriend and my best friend? To extract DNA for this activity, it is best to use mushy fruit. Therapeutic Lifestyle Changes for a Happier, Healthier and Longer Life. ma)e up the membra"es surrou"%#"g the ce$$ a"% "uc$eus. Scientists, teachers, writers, illustrators, and translators are all important to the program. As a human, you usually get a set of instructions from your mom and another set from your dad more, Molecule: a chemical structure that has two or more atoms held together by a chemical bond. However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. assignments. [/Pattern /DeviceRGB] This lab was conducted in order to show and analyze the way DNA is extracted. (Remove the skin of the bananas and kiwi, we just want the insides!) A water bath was prepared. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. Extraction solution S was added into ziplock Extraction 1 and extraxtion solution SS into ziplock bad Extraction 2. What does soap do when you wash your hands. /Pages 3 0 R Although nowadays very few errors are made due to scientists using biotechnology equipment that allows them to look at DNA to see exactly how organisms are different, and find out how they work. /ca 1.0 All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. PDF Artificial Life Viewing Activity Teacher Notes Extracting DNA from Bananas They produce molecules called proteins that do most of the work in the body. Free Samples and Examples of Essays, Homeworks and any Papers, Filed Under: Essays Tagged With: Genetics. 0000008815 00000 n MATERIALS AND APPARATUS PER CLASS * (60-70 oC) Water bath * 95% Ethanol * Extraction solution * Cheesecloth Ice chest containing ice PER GROUP * 40g Banana * 2 ziplock bags (Label Extraction 1 and Extraction 2) * 2 funnels * 2 test tubes * 50ml conical tube (2 pieces) * 500ml beaker (2 pieces) * Glass Rod (2 pieces) * Shampoo 4ml * Distilled water 40ml * Table Salt (NaOH) 0. This cookie is set by GDPR Cookie Consent plugin. /4 When looking at DNA extraction,the key steps for isolating DNA from a plant cell or animal cell is to the solution is minimal remove the banana residue and transfer 5mL of the filtrate into a separate dry gas tube. DNA Extraction Conclusion - Google Docs Spinach DNA Extraction REE: The results from our experiment have shown that we were not able to spool DNA from eukaryotic cells. "Seeing DNA". Need urgent help with your paper? We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. The soap breaks apart the cell membrane and the cell parts that hold the DNA. By volunteering, or simply sending us feedback on the site. PDF Dna Extraction Lab Report Banana Conclusion There are three basic steps in DNA extraction. 0tx AA~/''8szs' / J~tG x #. Studying DNA is one way scientists learn about what is necessary for life. All the methods employ agarose gel electrophoresis to separate the DNA molecules. Step 8: Pour the drained liquid into a glass. 135 32 Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. database? The Mendelian Revolution: The Emergence of Hereditarian Concepts in Modern Science and Society. 0000039741 00000 n DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. 0000000936 00000 n When you are done, take a closer look at the stuff on the stirrer. Video of this experiment can be found here: DN #s !ou"% #" the ce$$s o! Extracting quality DNA from scale insects, including mealybugs, can be difficult due to their small body size and waxy coating. /SMask /None>> endobj C q" If you are interested in helping with the website we have a Volunteers page to get the process started. In . Do not lower the bag or mashed the banana too close to the ZIP seal on the bag. Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . All of the above steps are completed in the same manner for the banana DNA extraction except for the adding of water during the smashing of the fruit. /Length 8 0 R xnFab5\8CA _4E$`i,yI:=x-Yd.5g:#6}^={s/0lH*|?x'}=]4}vb ~M4q'(OV%l`! 0000044671 00000 n During the DNA extraction, the soap pulls apart the fats (lipids) and proteins that make up the membranes surrounding the cell and nucleus. Analysis & Conclusion/Discussion - Jessica's RESEARCH PROJECT - Weebly /Type /Catalog 0000004435 00000 n First, the cell must be broken open to release the The four nitrogenous are A(adenine), G(guanine), T(thymine) and C(cytosine). p$ace #t #" a cappe% tube 0$$e% w#th a$coho$. PhDessay is an educational resource where over 1,000,000 free essays are (60 oC) 2. 0000045111 00000 n Salt is used at a high molarity due to the fact that it precipitates all of the proteins out. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". HSj@WcczG]A)-84H$$`;:4yofRSLGiqU"M l 8cjzR. Extracting Dna From A Banana Experiment | PDF - Scribd Question 16 options: All of the above Improve crop production Improve disease resistance Generate new, 1 ________ is seen when ABCDEFGHI becomes ABCFEDGHI. Then the bananas were mushed more. Try to avoid making too much foam. ", American Psychological Association. 6. These cookies track visitors across websites and collect information to provide customized ads. %PDF-1.4 % ASU - Ask A Biologist, Web. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. 9>U0c4S7FK9>>Ns^|t_g7]}|qwwQvvpaZEU2pOUAK2wb)m-]v| re$ease% !rom the ce$$. and animals have DNA (deoxyribonucleic acid). Lea being the main narrator, sat up on her knees, Inviting the audience In to listen and seemed more comforting and engaged, whereas Phil communicated with the audience with the. >> After stirring for five to ten minutes, the mixture of banana and shampoo was filtered by pouring it into the coffee filter and letting the solution drain for several minutes until there was approximately five milliliters of filtrate to test. endobj 10 What do we have in common with the banana plant? * Pragya Khanna. <<37B5D6E5CA566B46AF6D8E49138E4E7A>]>> DNA extraction Science Learning Hub At an absorption of 280 wavelengths, the DNA measured 3.802 using a 374.66 ng/ul sample. We also use third-party cookies that help us analyze and understand how you use this website. Publisher: Arizona State University School of Life Sciences Ask A Biologist. Watch biologist Melissa Wilson Sayres as she shows you step-by-step how to extract DNA from a banana. Once these membranes are broken apart, the DNA is released from the cell. 0000001162 00000 n An academic unit ofThe College of Liberal Arts and Sciences, You may need to edit author's name to meet the style formats, which are in most cases "Last name, First name.